HyMaNaRe (PID2023-150170OB-I00; PI: María Concepción Serrano; 250 k€)
This page is specifically devoted to the content and advances related to the development of the research project HyMaNaRe, a Reseach Project funded by the Agencia Española de Investigación from the Ministerio de Ciencia, Innovación y Universidades of Spain. Since September 1st, 2024 through August 31st 2027, we will devote effort to the development of novel regenerative medicine strategies applied to SCI and stands on three major interdisciplinary pillars: nanotechnology, neural repair and motor rehabilitation (Scheme). To accomplish this overall aim, the general objectives are:
- Production of soft and silica-reinforced hybrid bio-implants based on hydrogels containing magnetic nanoparticles.
- Controlled release of neuroactive cargos by means of magnetic fields according to the temporal evolution of SCI damage and repair.
- Examination of the degradation, biocompatibility and neuroregenerative capacity of the resulting bio-implants in-vitro (neural and non-neural cell cultures) and in-vivo (cervical C6 hemisection model of SCI in rats).
This scheme depicts the main work hypothesis for the project, including the design of therapeutic magnetic bio-implants for neural repair (modified from doi.org/10.1038/s41563-023-01760-5; doi.org/10.1038/s41467-023-44481-8; doi.org/10.3390/ijms232213833).
The specific objectives are:
OBJECTIVE 1. Design and development of novel theranostic regenerative implants for neural repair based on silica-reinforced natural hydrogels containing chitosan-coated IONPs with neurotherapeutic cargos.
OBJECTIVE 2. Optimization of the implant lifetime: Biodegradation studies including temporal evolution of the physicochemical parameters of the implants and their fabrication by bioprinting.
OBJECTIVE 3. Evaluation of the biocompatibility in vitro of the nanomedicines developed, with particular focus on oxidative stress responses including ferroptosis.
OBJECTIVE 4. Analysis of the effect of magnetic fields, static (constant and gradients) and dynamic, on neural cells (in-vitro) and tissues (in-vivo) and the impact of magnetic nanoparticles on the biological responses found.
OBJECTIVE 5. Study of the diagnostic and regenerative capability in vivo of the resulting magnetic bio-implants in combination with magnetic fields and motor training routines in experimental models of SCI in rats.